Cell cycle-dependent accumulation of a kinesin-like protein, KatB/C, in synchronized tobacco BY-2 cells |
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| Authors: | Hisayuki Mitsui Seiichiro Hasezawa Toshiyuki Nagata Hideo Takahashi |
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| Institution: | (1) Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Plant Sciences, Graduate School of Science, the University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Immunoblot analysis with antibodies prepared against highly purified recombinant truncated kinesin-like proteins, KatB(5–249) and KatC(207–754), encoded by the katB and katC genes of Arabidopsis thaliana revealed the presence of a kinesin-like polypeptide, termed KatB/C, in cultured tobacco BY-2 cells. The KatB/C polypeptide cosedimented with microtubules in the presence of a nonhydrolyzable ATP analogue and was released from microtubules in the presence of ATP, both of which are characteristics of kinesin proteins. The amount of KatB/C polypeptide in synchronous BY-2 cells increased during M phase of the cell cycle. Microtubule-based structures present in cells at M phase, such as the spindle and phragmoplast, may be the site of action of the KatB/C protein. |
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| Keywords: | cell cycle kinesin-like protein M phase tobacco BY-2 cell kat genes |
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