A Drosophila hsp70 gene contains long,antiparallel, coupled open reading frames (LAC ORFs) conserved in homologous loci |
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| Authors: | Irene Konstantopoulou Christos A. Ouzounis Elena Drosopoulou Minas Yiangou Paschalis Sideras Chris Sander Zacharias G. Scouras |
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| Affiliation: | (1) Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki (AUTH), GR-54006 Thessaloniki, Greece;(2) Protein Design Group, European Molecular Biology Laboratory, D-69012 Heidelberg, Germany;(3) Unit for Applied Cell and Molecular Biology, University of Umea, S-90187 Umea, Sweden |
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| Abstract: | A clone isolated from a Drosophila auraria heat-shock cDNA library presents two long, antiparallel, coupled (LAC) open reading frames (ORFs). One strand ORF is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of D. melanogaster, while the second strand ORF, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, NAD+-dependent glutamate dehydrogenase (NAD+-GDH). The overlap of the two ORFs is 1,824 nucleotides long. Computational analysis shows that this LAC ORF arrangement is conserved in other hsp70 loci in a wide range of organisms, raising questions about possible evolutionary benefits of such a peculiar genomic organization.Correspondence to: Z.G. Scouras |
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| Keywords: | Gene structure Heat shock hsp70 Antiparallel ORFs Drosophila |
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