Expression of GDF-9, BMP-15 and their receptors in mammalian ovary follicles |
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| Authors: | R Z Sun L Lei L Cheng Z F Jin S J Zu Z Y Shan Z D Wang J X Zhang Z H Liu |
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| Institution: | (1) Department of Histology and Embryology, Harbin Medical University, 150081 Harbin, China;(2) College of Animal Science and Medicine, Inner Mongolia Agricultural University, 010018 Huhhot, China;(3) Department of Forensic Medicine, Harbin Medical University, 150081 Harbin, China;(4) College of Life Sciences, Northeast Agricultural University, 150031 Harbin, China; |
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| Abstract: | The synergetic process of folliculogenesis is mainly regulated by GDF-9 and BMP-15 as well as their receptors, such as BMPR2,
TβR1 and BMPR1B. Expressions of these factors and the receptors are significant different among species. This study was designed
to detect expression of GDF-9, BMP-15 and their receptors in mouse, porcine and human healthy follicles by immunohistochemistry.
Three ages of human ovary were studied according to ovarian developmental schedule, i.e. gestational week (GW) 16, puberty
(14 year-old) and adult (40 year-old). The results showed that both GDF-9 and BMP-15 were detectable in oocytes from primary
follicles onward, besides, BMP-15 also presented in granulosa cells (GCs) and follicular follicle of mature follicles in mouse.
However, they were maintained in oocytes and GCs from primordial to mature follicles in porcine except that GDF-9 was undetectable
in GCs of mature follicles. For human ovary, GDF-9 presented in oocytes of primordial follicles in all samples, whereas BMP-15
was only observed in primordial follicle of adult ovary. Receptors, BMPR2, TβR1 and BMPR1B were found in oocytes and GCs of
all follicles in mouse and porcine. In human, they were stained in oocytes from primordial follices but BMPR1B was not expressed
in pubertal primordial follicles. Furthermore, we found that GDF-9, BMP-15 and three receptors distributed in adult corpus
lutea. Collectively, our studies suggested that GDF-9, BMP-15 and their receptors might correlate with primordial follicular
recruitment in pig and human. Positive expression of the receptors (BMPR2, TβR1 and BMPR1B)in primordial follicles of mouse
ovaries indicated that these receptors might interact with others ligands besides GDF-9 and BMP-15 to regulate primordial
follicular activity in mouse. Moreover, presence of GDF-9 in oocytes and BMP-15 in oocytes and GCs of mature follicles from
mice and porcine elucidated coordinated roles of GDF-9 and BMP-15 in cumulus oophorus expansion. Additionally, expression
of these factors in adult human corpus lutea suggested they play roles in corpus luteum activity. |
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