Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis |
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Authors: | Aimee M. Terauchi Graham Peers Marilyn C. Kobayashi Krishna K. Niyogi Sabeeha S. Merchant |
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Affiliation: | (1) Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1569, USA;(2) Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA;(3) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;(4) Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA; |
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Abstract: | To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO2-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-intuitively, more chlorophyll on a per cell basis. We found that phototrophic cells are less impacted by iron deficiency and this correlates with their higher iron content on a per cell basis, suggesting a greater capacity/ability for iron assimilation in this metabolic state. Phototrophic cells maintain both photosynthetic and respiratory function and their associated Fe-containing proteins in conditions where heterotrophic cells lose photosynthetic capacity and have reduced oxygen evolution activity. Maintenance of NPQ capacity might contribute to protection of the photosynthetic apparatus in iron-limited phototrophic cells. Acetate-grown iron-limited cells maintain high growth rates by suppressing photosynthesis but increasing instead respiration. These cells are also able to maintain a reduced plastoquinone pool. |
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