Lipopolysaccharides ofVibrio fluvialis 181-86 Kobe possessing an antigenic factor in common with O1Vibrio cholerae

The chemical and serological characteristics of lipopolysaccharides (LPS) isolated fromVibrio fluvialis 181-86 Kobe were compared with those of LPS isolated from O1Vibrio cholerae and Vibrio bio-serogroup 1875 (Original and Variant), a marine vibrio possessing an antigenic factor in common with O1Vibrio cholerae. Two kinds of LPS (S-type LPS and R-type LPS) were extracted fromV. fluvialis 181-86 Kobe. The S-type LPS is different from LPS of the other serotypes (O1–O18) ofV. fluvialis in that the former was found to contain the pair perosamine and quinovosamine, a characteristic component of O1V. cholerae LPS. Furthermore, the sugar composition of the S-type LPS is close to that of Vibrio bio-serogroup 1875 LPS because both LPS contained mannose and the same two unidentified neutral sugars. Definite serological cross-reactivity in the passive hemolysis and the passive hemolysis inhibition tests were demonstrated between LPS fromV. fluvialis 181-86 Kobe and that from Vibrio bioserogroup 1875 Variant.