| 黑爪异鲵LDH同工酶谱系的研究 |
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| 引用本文： | 徐晋麟,马学海.黑爪异鲵LDH同工酶谱系的研究[J].动物学报,1996,42(2):182-188. |
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| 作者姓名： | 徐晋麟 马学海 |
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| 作者单位： | 上海交通大学生物科学与技术系!上海200030(徐晋麟)，中国科学院上海生理研究所!上海200031(马学海) |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳对黑爪异鲵１２种组织的ＬＤＨ同工酶谱系进行了研究，酶谱呈５带型，但在雄性肝中出现了ＬＤＨ－Ｃ带，在精巢中未发现，相对迁移率为Ｂ＞Ａ＞Ｃ。 经比较分析，其ｌｄｈ－Ｃ基因表达的组织特异性和性别特异性不仅证实黑爪异鲵是小鲵科中一个较原始的类群，且表明ｌｄｈ－Ｃ基因这种表达调控方式在进化中为鱼类与鸟类。哺乳类之间的一种过渡类型。
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| 关 键 词： | 黑爪异鲵 两栖纲 小鲵科 乳酸脱氢酶 同功酶 |
LDH ISOZYMES PATTERNS OF XENOBIUS MELANONYCHUS |
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| XU JIN-LIN.LDH ISOZYMES PATTERNS OF XENOBIUS MELANONYCHUS[J].Acta Zoologica Sinica,1996,42(2):182-188. |
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| Authors: | XU JIN-LIN |
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| Abstract: | The patterns of expression of LDH isozymes of Xenobius melanonychus were investigat ed in 12 different adult tissue (heart, liver, stomach, intestine, spleen, lung, kidney, spermary, ovary, muscle, eye, tongue). Isozymes of Xenobius melanonychus were resolved by vertical slab PAGE. The results show that LDH isozymes in the adult exhibit a tissue-specific expression. The pattern of LDH isozymes had the following characteristics: 1 ) It showed a 5 band type; 2) The C band was expressed only in the liver of males; 3) The relative anodemobility (RAM) was B > A > C. These results show that X. melanonychus is a primeval group distinctive in the family Hynobiidae. It is a transitional type from fishes to birds and mammals expressed in C gene independent regulation and was first found in amphibians by this study. In the present paper, the evolution of ldh-C the genetic and molecular bases of some isozymes mentioned above in X. melanonychus were discussed. |
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| Keywords: | Xenobius melanonychus Isozymes ldh-C gene PAGE Evolution |
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