Synthesis of rat transferrin in Escherichia coli containing a recombinant bacteriophage |
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Authors: | A R Aldred G J Howlett G Schreiber |
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Affiliation: | Department of Animal Drug Metabolism Merck Sharp & Dohme Research Laboratories Rahway, New Jersey 07065 USA |
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Abstract: | Using mRNA from rat liver a cDNA library was constructed in lambda gt11Amp3. Immunochemical screening identified 15 clones producing transferrin. The identity of two clones was confirmed by nucleotide sequencing, which also indicated a presegment rich in hydrophobic amino acids but lack of a prosegment in precursor transferrin. A 920 base pair insert in one clone corresponded to 84% of the N-terminal domain of transferrin, which was synthesized as a hybrid protein with bacterial beta-galactosidase. A 1540 base pair insert in another clone corresponded to the N-terminal plus 50% of the carboxy terminal domain of transferrin. The product of this clone possessed only antigenic properties of transferrin. |
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