Urea Hydrolysis and Suppressed Production of Thermostable Direct Hemolysin (TDH) by Vibrio parahaemolyticus Associated with Presence of TDH-Related Hemolysin Genes |
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| Authors: | Tadayuki Okitsu Ro Osawa Srirat Pornruangwong Shiro Yamai |
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| Affiliation: | (1) Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratories, 1-1-1 Nakao, Asahi-ku, Yokohama-shi, Kanagawa, 241, Japan , JP;(2) Division of Clinical Pathology, National Institute of Health, Ministry of Public Health, Nonthaburi, 11000, Thailand , TH |
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| Abstract: | A total of 18 strains of V. parahaemolyticus isolated from patients of past food poisoning cases occurring in Kanagawa Prefecture, Japan, were assayed for presence of the thermostable direct hemolysin (TDH) gene and the TDH-related hemolysin (TRH) genes (trh 1 and trh 2) with specific reference to their ability to hydrolyze urea and TDH production. A polymerase chain reaction assay revealed that all urea-hydrolyzing strains (9 strains) carried either trh 1 gene or trh 2 gene. The strains carrying the trh genes as well as the tdh gene produced TDH less by a factor of 4 to 16 than those carrying only the tdh gene, suggesting the expression of the tdh gene was suppressed by the presence of trh gene through a mechanism yet to be defined. Received: 20 September 1996 / Accepted: 6 November 1996 |
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