Effect of O2 Concentration on Dark H2 Oxidation in Whole Cells of a Marine Sulfur Photosynthetic Bacterium (Chromatium sp. Strain Miami PBS 1071) |
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| Authors: | Kumazawa, Shuzo Ogawa, Teruo Inoue, Yorinao Mitsui, Akira |
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| Affiliation: | 1 Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN) Wako-shi, Saitama 351-01, Japan
2 School of Marine and Atomospheric Science, University of Miami Florida 33149, U.S.A. |
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| Abstract: | Whole cells of photoanaerobically grown Chromatium sp. strainMiami PBS 1071, a marine purple sulfur bacterium, oxidized H2in the dark through the oxyhydrogen reaction. Oxidation of H2was measured by injecting either H2 into an air-equilibratedcell suspension (microaerobic H2 oxidation) or O2 into an H2/Ar-equilibratedcell suspension (microaerobic H2 oxidation). Both types of H2oxidation were strongly inhibited by azide (40 mM), indicatingthat the oxidation proceeds via a terminal oxidase system. 2,5-Dibromo-3-methyl-6-isopropyl-p-benzoquinone(16 µM) inhibited aerobic H2 oxidation by 49% but it acceleratedmicroacrobic H2 oxidation. The sensitivity of H2 oxidation torotenone was higher under aerobic conditions. The results indicatethat H2 oxidation proceeds via two different pathways; one containsubiquinone and NAD, and the other does not. The contributionof each pathway depends on the O2 partial pressure. 4 Present address: Institute of Oceanic Research and Development,Tokai University, Shimizu, Shizuoka 424, Japan. (Received May 24, 1985; Accepted August 29, 1985) |
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