Murine monoclonal antibodies specific for lipopolysaccharide of Escherichia coli O26 and O111 |
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| Authors: | Rivera-Betancourt M Keen J E |
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| Affiliation: | Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933, USA. |
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| Abstract: | Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia coli O26 isolates and cross-reacted with 1 of 365 non-E. coli O26 isolates. MAb 15C4 reacted with 30 E. coli O111 strains and 8 Salmonella O35 strains (possessing identical O antigen) but not with 362 other bacterial strains. Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity. |
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