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Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae) |
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| Authors: | Sébastien J Puechmaille Piyathip Piyapan Medhi Yokubol Meriadeg Ar Gouilh Khin Mie Mie Paul J Bates Chutamas Satasook Tin Nwe Si Si Hla Bu Iain Mackie Emma C Teeling |
| Institution: | 1. School of Biological and Environmental Sciences, University College Dublin, Belfield, Dublin 4, Ireland 2. Department of Biology, Prince of Songkla University, Hat Yai, Songkhla, Thailand 3. Muséum National d’Histoire Naturelle, Paris, France 4. Faculty of Science, Mahidol University, Bangkok, Thailand 5. Zoology Department, Yangon University, Yangon, Myanmar 6. Harrison Institute, Bowerwood House, St Botolph’s Road, Sevenoaks, Kent, TN13 3AQ, UK 7. Department of Zoology, Mandalay University (formerly of Yangon University), Mandalay, Myanmar 8. Department of Biology, University of Aberdeen, IBES, Tillydrone Avenue, Aberdeen, UK |
| Abstract: | The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy–Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai. Tin Nwe, retired. |
| Keywords: | Craseonycteris thonglongyai Chiroptera Microsatellites Endangered species Population structure |
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