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Interspecific hybrid plant formation by electrofusion in Nicotiana |
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| Authors: | H Morikawa T Kumashiro K Kusakari A Iida A Hirai Y Yamada |
| Institution: | (1) Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606 Kyoto, Japan;(2) Iwata Experiment Station, Japan Tobacco Inc., Iwata, 438 Shizuoka, Japan;(3) Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University, 464 Nagoya, Japan |
| Abstract: | Summary We obtained complete hybrid plants by electrofusion of mesophyll protoplasts from Nicotiana glauca and N. langsdorffii. Electrofocusing analysis of Fraction I proteins isolated from the leaves of these plants confirmed their hybridity. Cytological analysis indicated that the chromosome number (2n) of these plants is between 60 to 66, suggesting that they are the products of triple fusion. All plants were fertile and set viable seeds after self pollination. As we did not use an AC field for electrofusion, the present results indicate that an AC field is not essential for obtaining hybrid plants with electrofusion. |
| Keywords: | Electrofusion Fertile somatic hybrid Fraction I protein analysis Nicotiana |
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