Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius |
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Authors: | Ann H Thompson David J Studholme Edward M Green David J Leak |
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Institution: | Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK. |
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Abstract: | Expression of a pyruvate decarboxylase (Pdc) pathway in metabolically versatile thermophilic bacteria could create novel ethanologenic organisms, but no suitable thermostable Pdc is available. We have demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillus thermoglucosidasius at up to 52 degrees C, while expression of Pdc polypeptides up to 54 degrees C was evident from Western blotting. By using an unstable lactate dehydrogenase (ldh) mutant of G. thermoglucosidasius, indirect evidence of Pdc activity in vivo was also obtained. |
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Keywords: | Decarboxylase Ethanol Geobacillus Pyruvate Thermophile |
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