Hydration of sodium hyaluronate, NMR study of an oriented sample |
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| Authors: | G Lahajnar A Rupprecht |
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| Institution: | 1. J. Stefan Institute, E. Kardelj University of Ljubljana, Ljubljana, Yugoslavia;2. University of Stockholm, Arrhenius Laboratory, Division of Physical Chemistry, S-106 91 Stockholm, Sweden;1. NORT, Aix-Marseille University, INRA, INSERM, 13005 Marseille, France;2. Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France;3. Adisseo France S.A.S., Center of Expertise and Research in Nutrition, 6 route Noire, 03600 Commentry, France;1. Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Kiel, Germany;2. Department of Plastic Surgery, University of Lübeck, Lübeck, Germany;1. School of Engineering and Computing Sciences, University of Durham, DH1 3LE, UK;2. School of Science and Technology, Middlesex University, The Burroughs, Hendon, London NW4 4BT, UK;3. The MathWorks, Matrix House, Cambridge CB4 0HH, UK;1. Center for Microbial Interface Biology, Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210, United States;2. Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH 43210, United States;3. Department of Chemical and Bimolecular Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, United States;4. Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina, 4211 Marsico Hall, 125 Mason Farm Road, Chapel Hill, NC 27599, United States;1. Department of Orthopedic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;2. Department of Neurology, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line width expressed by delta v o + A(1-3cos2 theta)2. This reveals a residual intramolecular dipolar interaction of anisotropically rotating water molecules. The merging of the expected doublet into a single line is assumed to be caused by proton exchange between water molecules. The result indicates a hydration similar to that of many other fibrous biopolymers at comparable relative humidities. |
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