Effects of MMP-9 inhibition by doxycycline on proteome of lungs in high tidal volume mechanical ventilation-induced acute lung injury |
| |
| Authors: | Adrian Doroszko Thomas S Hurst Dorota Polewicz Jolanta Sawicka Justyna Fert-Bober David H Johnson Grzegorz Sawicki |
| |
| Affiliation: | (1) Department of Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;(2) Department of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;(3) Department of Medicine, University of Alberta, Edmonton, Alberta, Canada;(4) Department of Clinical Chemistry, Medical University of Wroclaw, Wroclaw, Poland;(5) On leave of absence from Department of Internal Medicine and Hypertension, Wroclaw Medical University, Wroclaw, Poland |
| |
| Abstract: | Background Although mechanical ventilation (MV) is a major supportive therapy for patients with acute respiratory distress syndrome, it may result in side effects including lung injury. In this study we hypothesize that MMP-9 inhibition by doxycycline might reduce MV-related lung damage. Using a proteomic approach we identified the pulmonary proteins altered in high volume ventilation-induced lung injury (VILI). Forty Wistar rats were randomized to an orally pretreated with doxycycline group (n = 20) or to a placebo group (n = 20) each of which was followed by instrumentation prior to either low or high tidal volume mechanical ventilation. Afterwards, animals were euthanized and lungs were harvested for subsequent analyses. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
