Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens AM1 by use of a tetrahydromethanopterin bioassay |
| |
| Authors: | Rasche Madeline E Havemann Stephanie A Rosenzvaig Mariana |
| |
| Institution: | Microbiology and Cell Science Department, University of Florida, Gainesville, Florida 32611-0700, USA. mrasche@ufl.edu |
| |
| Abstract: | An enzymatic assay was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H(4)MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H(4)MPT biosynthesis. The protein encoded by orf4 catalyzed the reaction of ribofuranosylaminobenzene 5'-phosphate synthase, the first committed step of H(4)MPT biosynthesis. These results provide the first biochemical evidence for H(4)MPT biosynthesis genes in bacteria. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
