Effect of pH on Mn absorption by barley genotypes in a chelate-buffered nutrient solution |
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Authors: | Huang Chunyuan Webb Michael J. Graham Robin D. |
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Affiliation: | (1) Waite Agricultural Research Institute, 5064 Glen Osmond, South Australia |
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Abstract: | A new chelate-buffering technique was used to investigate the effect of pH (6.00, 6.85 and 7.70) on manganese (Mn) absorption from nutrient solution by three genotypes of barley plants differeing in Mn efficiency. The nutrient composition was adjusted such that the calculated activities of Mn2+, Zn2+, Cu2+ and Ni2+ were similar in each pH, thus eliminating any effect of the pH treatment on Mn2+ supply. Increasing pH from 6.00 to 7.70 increased the rate of Mn absorption and decreased the external Mn requirement for optimal growth rate. With increasing pH, Mn concentrations in roots rose markedly, and were higher than those in shoots at pH 7.70. Genotypic differences in Mn concentration of roots appeared only at higher pH. We suggest that higher Mn concentration in roots of inefficient plants may be related to Mn immobilisation in roots, and this may be a factor in the mechanism of Mn efficiency. |
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Keywords: | absorption barley chelate genotype HEDTA MES Mn pH Tris |
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