Disruption of a <Emphasis Type="Italic">fur</Emphasis>-like gene inhibits magnetosome formation in <Emphasis Type="Italic">Magnetospirillum gryphiswaldense</Emphasis> MSR-1 |
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Authors: | Huang Yijun Zhang Weijia Jiang Wei Rong Chengbo Li Ying |
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Institution: | (1) State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, 100094, China |
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Abstract: | In this study, a genomic library of Magnetospirillum gryphiswaldense MSR-1 strain was constructed and a fur-like gene (encoding Fur protein, ferric uptake regulator) was isolated and sequenced. This gene consisted of 420 bp and encoded
139 amino acid residues. To investigate the function of this gene in MSR-1, a fur mutant was generated by double crossover with a kanamycin cassette inserted into its coding region. Iron uptake and magnetosome
formation were dramatically inhibited by disruption of fur. Iron content analysis of the fur mutant indicated that it contained approximately 0.037% by dry weight, which was at least 10-fold less than that observed
in the wild type. Electron microscopy revealed the absence of a magnetosome in the fur mutant, although it was able to tolerate 1 mM H2O2 at 10-fold higher level than wild-type. These data suggest that Fur protein may possess a novel function in magnetic bacteria.
Published in Russian in Biokhimiya, 2007, Vol. 72, No. 11, pp. 1532–1539. |
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Keywords: | Magnetospirillum gryphiswaldense ferric uptake regulator (Fur) magnetosome formation |
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