Detection and properties of curvaticin 13, a bacteriocin-like substance produced byLactobacillus curvatus SB13 |
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| Authors: | I. Sudirman F. Mathieu M. Michel Prof. G. Lefebvre |
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| Affiliation: | (1) Laboratory of Industrial and Food Microbiology, National Superior School of Agronomy and Alimentary Industries, National Polytechnic Institute of Lorraine, 2 avenue de la Foret de Haye, 54500 Nancy, France |
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| Abstract: | Lactobacillus curvatus SB13 biosynthetized during growth and excreted into the culture medium an inhibitor active againstListeria monocytogenes, Staphylococcus aureus, and some other Gram-positive bacteria. The native activity was linked to a non-dialyzable proteinaceous complex, destroyed by proteases, with an apparent molecular weight greater that 10 kDa. It showed a hydrophobic character, being soluble in alcohols and not denaturated by several organic solvents. The molecule was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography. |
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