MEMBRANE REACTIONS DURING FERTILIZATION AND 1-METHYLADENINE STIMULATION IN IMMATURE STARFISH OOCYTES |
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| Authors: | HAROLD H LEE ELLEN GOLL MARY PLENZLER |
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| Institution: | Department of Biology, The University of Toledo, Toledo, Ohio 43606, U. S. A. |
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| Abstract: | Immature oocytes from starfishes were obtained during the breeding and non-breeding seasons. The oocytes were "fertilized" without previous induction of maturation by 1-methyladenine (1-MA) or germinal vesicle breakdown (GVB). Elevation of the fertilization membrane (FM) was observed with eggs obtained during the breeding season, but not observed with eggs obtained during the non-breeding season. Abnormal development was observed when these "fertilized" eggs with a FM were subsequently treated with 1-MA. The results of these experiments indicated that the elevation of FM was independent of GVB or the mixing of nuclear and cytoplasmic components. |
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