Single-nucleotide polymorphisms (SNPs) of the IRF6 and TFAP2A in non-syndromic cleft lip with or without cleft palate (NSCLP) in a northern Chinese population |
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Authors: | Shi Jinna Song Tao Jiao Xiaohui Qin Chunlin Zhou Jin |
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Affiliation: | aState Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;bKey Laboratory of MOE for Developmental Genetics and Neuropsychiatric Diseases, Shanghai Jiao Tong University, Shanghai 200240, China |
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Abstract: | The conserved armadillo repeat (ARM) domain of adenomatous polyposis coli (APC) protein plays an important role in the recognition of its binding partners. In this study, we report the crystal structure of APC-ARM (residues 407-775), which was determined to 2.9 Å resolution. Our structure shows that the seven armadillo repeats of APC-ARM fold together into a compact domain, with Arm2 and Arm5 presenting some deviations from canonical armadillo repeats. There is a positively charged groove on the surface of APC-ARM, which might be the recognition site for APC-binding partners. Comparison of this structure with our previously reported structure of APC (407-751), together with normal mode analysis, reveals that the APC-ARM domain possesses a limited intrinsic flexibility. We propose that this intrinsic flexibility might be an inherent property of ARM domains in general. |
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Keywords: | Adenomatous polyposis coli APC Armadillo repeat domain ARM domain Crystal structure Inherent flexibility |
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