Effect of ouabain on the lactogenic action of prolactin and on the level of mammary prolactin receptors |
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Authors: | L M Houdebine J Djiane |
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Affiliation: | Laboratoire de Physiologie de la Lactation, Institut National de la Recherche Agronomique, C.N.R.Z., 78350 Jouy-en-Josas, France |
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Abstract: | Ouabain added to the culture medium of rabbit mammary gland inhibits prolactin action on the initiation of lactose and casein synthesis. The degree of inhibition is a function of the ouabain concentration in the medium. Likewise, ouabain blocks the accumulation of casein mRNA supported by prolactin. In addition, ouabain provokes a rapid disappearance of prolactin receptors. Conversely prolactin keeps its capacity to enhance the concentration of casein mRNA and the parallel casein synthesis when K+ ions are totally absent from the culture medium. These results suggest that although prolactin induces a modification of the K+/Na+ ratio in the mammary cell and ouabain prevents this effect of prolactin, the inhibitory action of ouabain on lactogenesis can be explained essentially by its effect on the hormone receptors. |
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Keywords: | Ouabaïne Ion potassium Prolactine Synthèse des caséines RNA messagers des caséines Récepteurs prolactiniques Glande mammaire de lapine Ouabain Potassium ion Prolactin Casein synthesis Messenger RNA Prolactin receptor Rabbit mammary gland |
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