Production and structure elucidation of di- and oligosaccharide lipids (biosurfactants) from Tsukamurella sp. nov. |
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| Authors: | E Vollbrecht R Heckmann V Wray M Nimtz S Lang |
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| Institution: | Technische Universit?t Braunschweig, Institut für Biochemie und Biotechnologie, Abt. Biotechnologie, Spielmannstra?e 7, D-38106 Braunschweig, Germany Tel.: +49-531-3915737 Fax: +49-531-3915763, DE
Gesellschaft für Biotechnologische Forschung mbH, Abt. Molekulare Strukturforschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany, DE
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| Abstract: | The bacterium Tsukamurella sp. nov., isolated from soil, was found to produce novel glycolipids when grown on sunflower oil as the sole carbon source.
The glycolipids were isolated by chromatography on silica columns and their structures elucidated using a combination of multidimensional
NMR and MS techniques. The three main components are 2,3-di-O-acyl-α-d-glucopyranosyl-(1-1)-α-d-glucopyranose, 2,3-di-O-acyl-β-d-glucopyranosyl-(1-2)-4,6-di-O-acyl-α-d-glucopyranosyl-(1-1)-α-d-glucopyranose and 2,3-di-O-acyl-β-d-glucopyranosyl-(1-2)-β-d-galactopyranosyl-(1-6)-4,6-di-O-acyl-α-d-glucopyranosyl-(1-1)-α-d-glucopyranosl which are linked to fatty acids varying in chain length from C4 to C18. The glycolipids are mainly extracellular but are also found attached to the cell walls. During the cultivation the composition
of the glycolipids changed from disaccharide- to tri- and tetrasaccharide lipids. The glycolipids show good surface-active
behaviour and have antimicrobial properties.
Received: 22 May 1998 / Received revision: 24 August 1998 / Accepted: 26 August 1998 |
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