基因芯片与植物基因差异表达分析

基因芯片为研究植物不同个体或物种之间以及同一个体在不同生长发育阶段、正常和疾病状态下基因表达的差异、某一性状多基因的协同作用,寻找和定位新的目的基因等方面带来了革命性的变革。与传统研究基因差异表达的方法相比,它具有微型化、用材少、快速、准确、灵敏度能高基、在因同等一研究方面已取得了显著的成绩,如拟南芥、酵母、水稻等。

DNA MICROARRAY AND ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN PLANT

DNA microarray technology has made a revolutionary transformation in studying differential gene expression, the collaboration of multigene for one trait, discovering and locating target genes, among different individual in a same or different species and one plant individual in different development stage as well as between normal and diseased condition. The major advance of DNA microarray technology, as compared to conventional techniques, results from the small size of the array, which allows for a higher accurate, a quicker process and a higher sensitivity enables the parallel screening of larger number of genes simultaneously on one chip and provides the opportunity to use smaller amounts of starting material. Many significant achievements have been obtained in picturing the expression profiles of genes, discoverying the target genes and elucidating gene functions with DNA microarray on plant, such as Arabidopsis and yeast,rice.