Identification of the cytokinins in red pine seedlings

Zeatin-9-riboside was identified in shoots and roots of Pinus resinosa by GC-MS analysis of its permethyl derivative. Based on their chromatographic properties on Sephadex LH-20 and C₁₈ HPLC, and their susceptibility to enzymatic degradation, several other cytokinins have been tentatively identified. The basic fraction of both the roots and shoots contained zeatin, whereas the shoots contained dihydrozeatin-O-glucoside and the roots contained zeatin-O-glucoside. Zeatin-9-riboside monophosphate, isopentenyladenosine monophosphate ([9R-5P]iP) and glucosyl phosphate derivatives were detected in the acidic fractions from both roots and shoots. There were equivalent amounts of [9R-5P]iP in both roots and shoots. The presence of equivalent amounts of [9R-5P]iP in both the roots and shots suggests that cytokinin biosynthesis may be occurring in both locations.Abbreviations AMP adenosine-5-monophosphate - BAP benzylaminopurine - BSA bovine serum albumin - BuOH butan-1-ol - CK cytokinin - (diH)Z dihydrozeatin - (diH OG)Z dihydrozeatin-O-glucoside - (diH OG)[9R]Z dihydrozeatin-9-riboside-O-glucoside - DW dry weight - EtOH ethanol - FW fresh weight - GC-MS gas chromatography-mass spectrometry - HPLC high-performance liquid chromatography - [9R]iP isopentenyladenosine - [9R-5P]iP isopentenyladenosine monophosphate - MeOH methanol - PVP polyvinylpyrrolidone - RFE rotary film evaporation - TEAB triethyl ammonium bicarbonate - Z zeatin - [9R]Z zeatin-9-riboside - (OG)Z zeatin-O-glucoside - [7G]Z zeatin-7-glucoside - [9R-5P]Z zeatin-9-riboside monophosphate