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Impact of heparin-binding domain of recombinant human osteocalcin-fibronectinIII9-14 on the osteoblastic cell response |
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| Authors: | Ye-Rang Yun Hae-Won Kim Jun-Hyeog Jang |
| Institution: | 1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, South Korea 2. Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714, South Korea 3. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, South Korea 4. Department of Biochemistry, Inha University School of Medicine, Inchon, 400-712, South Korea |
| Abstract: | Fibronectin (FN) containing a heparin-binding domain (HBD) and an Arg-Gly-Asp (RGD) domain can promote cell adhesion and proliferation compared to FN that contained only RGD. Here, we have engineered recombinant human osteocalcin (rhOC) with FN type III9-14 (rhOC-FNIII9-14) containing RGD and HBD to promote the cellular activity of MC3T3-E1 cells, including adhesion, proliferation, and differentiation. RhOC-FNIII9-14 significantly increased cell adhesion and proliferation of MC3T3-E1 cells compared to rhOC-FNIII9-10 (P < 0.05). Moreover, rhOC-FNIII9-14 showed osteogenic differentiation of MC3T3-E1 cells in mineralization activity and osteogenic gene expression. |
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