Industrial-scale production and purification of a heterologous protein in <Emphasis Type="Italic">Lactococcus lactis</Emphasis> using the nisin-controlled gene expression system NICE: The case of lysostaphin期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Industrial-scale production and purification of a heterologous protein in <Emphasis Type="Italic">Lactococcus lactis</Emphasis> using the nisin-controlled gene expression system NICE: The case of lysostaphin

Authors:	Email author" target="_blank">Igor?Mierau Email author Peter?Leij Iris?van Swam Barry?Blommestein Esther?Floris James?Mond Eddy?J?Smid

Institution:	(1) NIZO food research, P.O. Box 20, 6710, BA Ede, The Netherlands;(2) Biosynexus, Inc., 20877, 9119 Gaither Road, Gaithersburg, MD, USA

Abstract:	Background The NIsin-Controlled gene Expression system NICE of Lactococcus lactis is one of the most widespread used expression systems of Gram-positive bacteria. It is used in more than 100 laboratories for laboratory-scale gene expression experiments. However, L. lactis is also a micro-organism with a large biotechnological potential. Therefore, the aim of this study was to test whether protein production in L. lactis using the NICE system can also effectively be performed at the industrial-scale of fermentation.

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