Effect of high-lysine mutations on the protein fractions of barley grain |
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Authors: | P R Shewry Audrey J Faulks B J Miflin |
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Institution: | (1) Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, England |
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Abstract: | We have studied the effects of six high-lysine barley mutations (Risø mutants 1508 and 56, Notch 1 and 2, lys 95 and 449) on the protein fractions of the grain. All mutants had a decreased relative and total amount of the lysine-poor hordein fraction, but only in Risø 1508 and 56 was the polypeptide composition of this fraction greatly affected. In all the mutants there were increases in the lysine-rich glutelin proteins and in nonprotein N while in Risø 1508 and the Notch mutants the total amount of salt-soluble proteins was also increased and their relative polypeptide composition substantially altered.This work was supported by EEC Contract No. 473 under the common program on plant protein improvement. |
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Keywords: | barley high-lysine mutants seed proteins electrophoresis |
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