Carrier-mediated acetate uptake in Corynebacterium glutamicum |
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Authors: | Holger Ebbighausen Brita Weil Reinhard Krämer |
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Affiliation: | (1) Institut für Biotechnologie, Forschungszentrum Jülich, Postfach 1913, W-5170 Jülich, Germany |
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Abstract: | Acetate is effectively taken up by whole cells of Corynebacterium glutamicum via a specific carrier with a pH optimum of 8. The K m of acetate uptake was 50 μM and the V max 25–35 nmol/mg dw min. The activation energy was determined to be 70 kJ/mol. Acetate uptake was competitively inhibited by propionate with a K i of about 30 μM and blocked by addition of sulfhydryl reagents. The transport activity was clearly dependent on the membrane potential, but independent of the presence of Na+-ions. It is concluded that uptake of acetate proceeds by a secondary, proton coupled mechanism. |
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Keywords: | Acetate transport-uptake Corynebacterium glutamicum Secondary transport |
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