High-performance liquid chromatographic determination of 6-aminopenicillanic acid by postcolumn alkaline degradation |
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| Authors: | J Haginaka J Wakai |
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| Institution: | 1. Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran;2. Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran;3. Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran;1. Key Laboratory of Urban Environment and Health, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China;2. College of Chemical Engineering, Northeast Electric Power University, Jilin 132012, China;3. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: | A high-performance liquid chromatographic method has been developed for the determination of 6-aminopenicillanic acid in amino acid mixtures and human serum. The separation of 6-aminopenicillanic acid was carried out on a C18 column using sodium heptylsulfonate or tetrabutylammonium bromide as an ion-pairing agent and methanol as an organic mobile phase modifier. Detection was based on a postcolumn reaction with sodium hydroxide, mercury(II) chloride, and ethylenediaminetetraacetic acid disodium salt followed by measurement of ultraviolet absorbance (at 300 nm) of the reaction product(s). The method is quantitative for 6-aminopenicillanic acid concentrations down to 0.1 microgram/ml in human serum samples with a 20-microliter injection. At a concentration of 2 micrograms/ml, the within- and between-run precisions (relative standard deviation) were 1.29-3.91% and 2.30%, respectively. |
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