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Cloning and analysis of a defender against apoptotic cell death (DAD1) homologue from tomato
Authors:Frank A. Hoeberichts  Ernst J. Woltering  
Affiliation:aAgrotechnological Research Institute (ATO), Wageningen University and Research Centre, Bornsesteeg 59, 6708 PD Wageningen, The Netherlands
Abstract:A cDNA clone homologous to the human defender against apoptotic cell death (DAD1) gene, which is believed to be a conserved inhibitor of programmed cell death, was isolated from tomato (Lycopersicon esculentum cv. Prisca). The 351 basepairs open reading frame predicted a 116 amino acid protein sequence (LeDAD1) that showed high homology to other DAD1 proteins. Northern analysis revealed that LeDAD1 was constitutively expressed during ripening of wildtype, rin,andNr tomato fruit.
Keywords:apoptosis   DAD1   Lycopersicon esculentum   programmed cell death   tomato fruit
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