Characterization of microbial community in an aerobic moving bed biofilm reactor applied for simultaneous nitrification and denitrification |
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Authors: | Bo Fu Xiaoyi Liao Lili Ding Hongqiang Ren |
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Institution: | (1) State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 210093 Nanjing, China; |
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Abstract: | A continuous-flow moving bed biofilm reactor (MBBR) under aerobic conditions was established for simultaneous nitrification
and denitrification (SND), and microbial communities were investigated by a combination of denaturing gel gradient electrophoresis
(DGGE) and fluorescence in situ hybridization (FISH). DGGE analysis has revealed more similar microbial community structures
formed in the biofilms with more similar carbon nitrogen (C/N) ratios. FISH analysis shows that the dominance of both Betaproteobacteria ammonia-oxidizing bacteria and Nitrospira-like nitrite-oxidizing bacteria were negatively correlated to C/N ratios. Sequence analysis of DGGE bands has indicated the
presence of anoxic denitrifying bacteria Agrobacterium tumefaciens and Rhizobium sp., suggesting that the oxygen gradient inside the biofilm may be responsible for the mechanism of SND in aerobic MBBRs. The
study confirms that appropriate control of microbial community structure resulting from optimal C/N ratio is beneficial in
improving SND, thus optimizing nitrogen removal in aerobic MBBR. The established SND-based MBBR can save operation space and
time in comparison to the traditional nitrogen removal process, and might be very attractive for future practical applications. |
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Keywords: | |
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