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| 磷脂酶Dδ参与植物的低温驯化过程 | |
| 引用本文： | 王涛,梅旭荣,钟秀丽,李玉中,曾正兵,王海燕,孙磊,夏旭. 磷脂酶Dδ参与植物的低温驯化过程[J]. 植物学报, 2010, 45(5): 541-547. DOI: 10.3969/j.issn.1674-3466.2010.05.003 |
| 作者姓名： | 王涛 梅旭荣 钟秀丽 李玉中 曾正兵 王海燕 孙磊 夏旭 |
| 作者单位： | 1中国农业科学院农业环境与可持续发展研究所, 农业部旱作节水农业重点开放实验室, 北京 100081 2云南瑞升烟草技术(集团)有限公司, 昆明 650106 |
| 摘 要： | 对经低温驯化和未经低温驯化的磷脂酶Dδ (PLDδ)基因敲除突变体与野生型植株进行冻害胁迫处理后, 比较2种基因型植株的抗冻性。结果发现, 经低温驯化的PLDδ敲除突变体的抗冻性明显低于野生型, 而未经低温驯化的PLDδ敲除突变体与野生型的抗冻性没有显著差异, 表明PLDδ参与植物的低温驯化过程。对PLDδ的作用途径进行分析, 发现PLDδ在低温驯化过程中不参与抗氧化酶活性的调节, 对脯氨酸和可溶性糖的积累起负调节作用, 但是参与低温信号转导物质ABA诱导抗冻性的过程。 |
| 关 键 词： | 低温驯化 磷脂酶Dδ 信号转导 |
| 收稿时间： | 2009-10-23 |
| 修稿时间： | 2010-01-05 |
Phospholipase Dδ Mediates the Process of Cold Acclimation in Arabidopsis thaliana |
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| Tao Wang,Xurong Mei,Xiuli Zhong,Yuzhong Li,Zhengbing Zeng,Haiyan Wang,Lei Sun,Xu Xia. Phospholipase Dδ Mediates the Process of Cold Acclimation in Arabidopsis thaliana[J]. Bulletin of Botany, 2010, 45(5): 541-547. DOI: 10.3969/j.issn.1674-3466.2010.05.003 | |
| Authors: | Tao Wang Xurong Mei Xiuli Zhong Yuzhong Li Zhengbing Zeng Haiyan Wang Lei Sun Xu Xia |
| Affiliation: | 1Key Laboratory of Dryland Farming and Water-saving Agriculture, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming 650106, China |
| Abstract: | We compared cold-acclimated and non-acclimated phospholipase D (PLD)δ-knockout and wild-typeArabidopsis thaliana plants for tolerance to freezing. PLDδ-knockout plants showed lower freezing tolerance than did wild-type plants with cold acclimation; without cold acclimation, the two genotypes did not differ in freezing tolerance. Therefore, the PLDδ gene is involved in the process of cold acclimation. The pathways through which PLDδ acts in the process of cold acclimation were then analyzed. During cold acclimation, PLDδ was not involved in the activation of SOD, CAT, or POD anti-oxidative enzymes; it negatively regulated the accumulation of proline and soluble sugar, and mediated the process of freezing resistance induced by the low temperature signaling molecule ABA. |
| Keywords: | cold acclimation phospholipase D&delta signaling |
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