Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens# |
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Authors: | Fangui Min Yu Zhang Jinchun Pan Jing Wang Wen Yuan |
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Institution: | 1)Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P. R. China |
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Abstract: | Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals
and human who come into contact with them, while the rapid, accurate, and robust diagnosis
is challenging. In this study, we first sought to establish an appropriate primate TB
model resembling natural TB in nonhuman primates. Four rhesus monkeys (Macaca
mulatta) of Chinese origin were infected intratracheally with two low doses of
M. tuberculosis H37Rv. Regardless of the infectious doses, all monkeys
were demonstrated to be successfully infected by clinical assessments, tuberculin skin
test conversions, peripheral immune responses, gross observations, histopathology
analysis, and M. tuberculosis burdens. Furthermore, we extended the
usefulness of this model for assessing the following immunodiagnostic antigens: CFP10,
ESAT-6, CFP10-ESAT-6, and an antigen cocktail of CFP10 and ESAT-6. The data showed that
CFP10 was an M. tuberculosis-specific, “early” antigen used for
serodiagnosis of TB in nonhuman primates. In conclusion, we established a useful primate
TB model depending on low doses of M .tuberculosis and affording new
opportunities for studies of M. tuberculosis disease and diagnostics. |
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Keywords: | infection Mycobacterium tuberculosis rhesus monkeys (Macaca mulatta) serodiagnostic antigen |
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