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Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies |
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| Institution: | Department of Biology, College of Science and Arts at Khulis, University of Jeddah, Jeddah, Saudi Arabia |
| Abstract: | Testicular function and structure harmed by ageing. Goal of this research was to assess preventive actions of soy isoflavone oral administration for 8 weeks on testes of old male albino rats, and potential mechanisms of action. Adult control (N = 10) and elderly control (N = 10) rats were fed usual diet, while aged treatment group (N = 10) gave oral 100 mg/kg soy isoflavone daily for 8 weeks. ELISA kits were used to measure testosterone levels and oxidative stress indicators malonaldehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD)] in serum. Aging produced functional and structural testicular changes and decreased ki67 proliferative marker immunoexpression versus adult control rats due to enhancement of oxidative stress. Soy isoflavone exerted protective effect on testicular function and structure as assessed by increase serum levels of testosterone and preserved histological structure and immune-expression features. These protected effects due to isoflavone antioxidant properties proved by decrease in serum values of MDA, while GSH and SOD were elevated after treatment. These data demonstrated protective effects of isoflavone against age changes in rat testes, by reducing oxidative stress and increasing antioxidants and testicular ki67 proliferative marker immunoexpression. |
| Keywords: | Aging Ki-67 testicular immunoexpression Testicular functions Oxidative stress Rat Soy isoflavone ELISA"} {"#name":"keyword" "$":{"id":"pc_QIJDh2xICI"} "$$":[{"#name":"text" "_":"The enzyme-linked immunosorbent assay MDA"} {"#name":"keyword" "$":{"id":"pc_tsQfn2NVQ8"} "$$":[{"#name":"text" "_":"malonaldehyde GSH"} {"#name":"keyword" "$":{"id":"pc_6jM7ev9t9V"} "$$":[{"#name":"text" "_":"glutathione SOD"} {"#name":"keyword" "$":{"id":"pc_1fEugFp2SR"} "$$":[{"#name":"text" "_":"superoxide dismutase T"} {"#name":"keyword" "$":{"id":"pc_MsjZVAzEwG"} "$$":[{"#name":"text" "_":"testosterone SI"} {"#name":"keyword" "$":{"id":"pc_pTNiu2KVTT"} "$$":[{"#name":"text" "_":"Soybean isoflavones ANOVA"} {"#name":"keyword" "$":{"id":"pc_j2rGt7MzFv"} "$$":[{"#name":"text" "_":"Analysis of variance LSD"} {"#name":"keyword" "$":{"id":"pc_aIKfJvdv36"} "$$":[{"#name":"text" "_":"least significant difference P"} {"#name":"keyword" "$":{"id":"pc_do9Z7E2nRn"} "$$":[{"#name":"text" "_":"P-value DHT"} {"#name":"keyword" "$":{"id":"pc_3M763k5nXi"} "$$":[{"#name":"text" "_":"dihyrotestosterone CAT"} {"#name":"keyword" "$":{"id":"pc_tvlHMOsYnC"} "$$":[{"#name":"text" "_":"catalase GST"} {"#name":"keyword" "$":{"id":"pc_FvizzOCAy5"} "$$":[{"#name":"text" "_":"glutathione transferase GPx"} {"#name":"keyword" "$":{"id":"pc_hNT34AS21l"} "$$":[{"#name":"text" "_":"glutathione peroxidase |
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