Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells |
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| Authors: | Yan-fang Zhao Jing Xu Wen-juan Wang Jin Wang Juan-wen He Li Li Qian Dong Yan Xiao Xing-lian Duan Xue Yang Yi-wen Liang Tao Song Min Tang Dan Zhao Jin-yong Luo |
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| Institution: | Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, PR China |
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| Abstract: | Although BMP9 is highly capable of promoting osteogenic differentiation of mesenchymal stem cell (MSCs), the molecular mechanism involved remains to be fully elucidated. Here, we explore the possible involvement and detail role of JNKs (c-Jun N-terminal kinases) in BMP9-induced osteogenic differentiation of MSCs. It was found that BMP9 stimulated the activation of JNKs in MSCs. BMP9-induced osteogenic differentiation of MSCs was dramatically inhibited by JNKs inhibitor SP600125. Moreover, BMP9-activated Smads signaling was decreased by SP600125 treatment in MSCs. The effects of inhibitor are reproduced with adenoviruses expressing siRNA targeted JNKs. Taken together, our results revealed that JNKs was activated in BMP9-induced osteogenic differentiation of MSCs. What is most noteworthy, however, is that inhibition of JNKs activity resulted in reduction of BMP9-induced osteogenic differentiation of MSCs, implying that activation of JNKs is essential for BMP9 osteoinductive activity. BMB Reports 2013; 46(8):422-427] |
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| Keywords: | BMP9 Bone morphogenetic proteins Mesenchymal stem cells Mitogen activated protein kinases Osteogenic differentiation |
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