The role of RAR1 in Agrobacterium-mediated plant transformation |
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Authors: | Ajith Anand Kirankumar S Mysore |
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Affiliation: | Plant Biology Division; Samuel Roberts Noble Foundation; Ardmore, OK USA |
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Abstract: | RAR1 is identified as a critical protein involved in plant innate immunity. We investigated the role of RAR1 in Agrobacterium-mediated plant transformation based on the previous findings that accessory proteins associated with the E3 ligase complex such as SGT1, which tightly interacts with RAR1, play a role in the transformation process. RAR1 gene silencing in Nicotiana benthamiana and Arabidopsis rar1 mutant analysis suggested that RAR1 is required for early stages of Agrobacterium-mediated plant transformation. This finding further illustrates that RAR1, along with SGT1, that serve as a HSP90 co-chaperone is important for Agrobacterium-mediated plant transformation. |
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Keywords: | Agrobacterium F-box proteins plant transformation SCF-E3 ligase VIGS |
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