膜片箝pClamp采样软件中的P/N漏减分析

本文采用大鼠海马脑片盲法的全细胞记录技术,研究了Ａｘｏｎ公司ｐＣｌａｍｐ采样软件Ｌｌａｍｐｅｘ中Ｐ／Ｎ漏减的功能意义和作用机制,对Ｐ／Ｎ漏减脉冲电流的采集以及漏减脉冲的时间、极性、箝位电压、数目、位置等参数的设置进行了详细分析。结果表明,在采集电压门控性离子通道电流时,合理地利用Ｐ／Ｎ漏减会使离子电流记录更为准确可靠。

Analysis of P/N leak subtraction of pClamp acquisition software

Using blind whole cell recording technique on rat hippocampal slices, the function and mechanisms of P/N leak subtraction of Clampex program in pClamp acquisition software were studied. The ways of picking up sub-pulse current and properly selecting parameters, including time course, polarization, sub-holding potential, numbers and location of P/N sub-pulse were analyzed in detail. Our results indicate that voltage-gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction.