大壁虎不同地理居群的遗传变异与分化

以线粒体细胞色素b(Cytb)基因作为分子标记,对中国广西12个地区,以及越南和老挝大壁虎(Gekko gecko)进行序列测定,获得Cytb基因424bp的序列片段,共有7个单倍型。以白脊壁虎和沙虎为外群,用邻接法和最大简约法构建了大壁虎不同地理种群的系统发育关系,其结果显示中国广西4个不同单倍型黑大壁虎之间的平均遗传距离为0.20%—1.20%,越南红大壁虎与老挝红大壁虎之间的平均遗传距离为0.50%,广西宁明红大壁虎与越南红大壁虎和老挝红大壁虎之间平均遗传距离分别为1.70%和2.20%。广西黑大壁虎种群与红大壁虎种群之间的平均遗传距离为8.60%—9.50%,达到了亚种或种分化的差异。

Genetic Variation and Differentiation of Gekko gecko from Different Population

Samples of Gekko gecko were collected from 12 areas of Guangxi China,Vietnam and Laos.Four hundred and twenty four base pairs of G.gecko mitochondrial Cyt b gene were sequenced and seven haplotypes were found.Gekko vittatus and Teratoscincus keyserlingii acting as outgroups,the phylogenetic trees of the G.gecko constructed using NJ and MP methods showed that the average genetic distances were:0.20%-1.20% between four haplotypes of black G.gecko from Guangxi China;0.50% between Vietnamese and Laotian red G.gecko;1.70% between Ningming and Vietnamese red G.gecko;2.20% between Guangxi and Laotian red G.gecko;8.60%-9.50% between black G.gecko from Guangxi and all red G.gecko.The genetic difference of black and red G.gecko reached the species or subspecies differentiation.