Enzymatic production of L-tryptophan in liquid membrane systems |
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| Authors: | D K Eggers D J Lim H W Blanch |
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| Institution: | (1) Department of Chemical Engineering, University of California, 94720 Berkeley, California, USA;(2) Department of Industrial Chemistry, Yeungnam University, 632 Gyongsan, Korea |
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| Abstract: | Tryptophan synthesis was investigated in a two-phase system employing an organic liquid membrane. A diffusion cell was constructed to study the transport of the various components of the reaction through an organic layer of cyclohexane. The organic phase was supported by two polymeric membranes, and Aliquat-336 was used as the anion exchanger. A differential in pH was maintained between the aqueous phases to facilitate extraction of the product from the reaction phase. A mathematical model was developed to estimate effective diffusivities and predict the sensitivity of the system to changes in the partition coefficients and liquid membrane thickness. The use of liquid membrane emulsion-type reactors is discussed. |
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