Genetic studies with a phosphoglucose isomerase mutant of Saccharomyces cerevisiae. |
| |
Authors: | P. K. Maitra and Zita Lobo |
| |
Affiliation: | (1) Tata Institute of Fundamental Research, 400005 Bombay, India |
| |
Abstract: | Summary A mutation pgi1 in the yeast Saccharomyces cerevisiae conferring deficiency of the glycolytic enzyme glucose 6-phosphate isomerase is characterised genetically. The mutation segregates 2+:2- in tetrads from diploids heterozygous for the mutant phenotype. The mutation is semi-dominant and is located on the right arm of chromosome II in the order: tsm134-lys2-pgi1-tyr1 approximately 15 map units from tyr1. The mutation pgi1 defines the structural gene of glucose 6-phosphate isomerase and can be suppressed intragenically giving revertants that have an unstable enzyme. In one temperature-sensitive revertant no enzyme activity in excess of the mutant level could be detected although fructose 6-phosphate was converted to glucose 6-phosphate in vivo. The suppressor locus in this revertant is dominant and is unlinked to the pgi1 locus. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |