NB-506, an indolocarbazole topoisomerase I inhibitor, binds preferentially to triplex DNA |
| |
Authors: | Ren J Bailly C Chaires J B |
| |
Affiliation: | Department of Biochemistry, University of Mississippi Medical Center, 2500 N. State St., Jackson, MS 39216-4505, USA. |
| |
Abstract: | A novel competition dialysis method was used to study the structural selectivity of the nucleic acid binding of NB-506, a promising indolocarbazole anticancer agent. A pronounced preference for NB-506 binding to the DNA triplex poly [dA]:(poly[dT])(2) was observed among potential binding to 12 different nucleic acid structures and sequences. Structures included in the assay ranged from single-stranded DNA, through a variety of right-handed DNA duplexes, to multistranded triplex and tetraplex forms. RNA and left-handed Z DNA were also included in the assay. The preferential binding to triplex was confirmed by UV melting experiments. The novel and unexpected structural selectivity shown by NB-506 may arise from a complementary shape between its extended aromatic ring system and the planar triplex stack. |
| |
Keywords: | DNA RNA Intercalation Dialysis Anticancer drug Triplex DNA |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|