Cloning, expression and purification of the DNA binding domain of RFX protein |
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| Authors: | Panchal S C Madan A Hosur R V |
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| Affiliation: | Department of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai. |
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| Abstract: | The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed. |
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