Nitric oxide synthase activity and the level of nitrates/nitrites in brain regions during spontaneous morphine withdrawal in rats |
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Authors: | D. I. Peregud M. V. Onufriev A. A. Yakovlev M. Yu. Stepanichev N. A. Lazareva T. V. Pavlova L. F. Panchenko N. V. Gulyaeva |
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Affiliation: | (1) National Scientific Center on Addiction, Ministry of Healthcare of Russian Federation, M. Mogiltsevskij per. 3, Moscow, 119002, Russia;(2) Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia;(3) Moscow Institute of Physics and Technology, Dolgoprudnyi, Moscow region, Russia |
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Abstract: | Activity of nitric oxide synthase (NOS) and concentrations of nitrate/nitrites (NO x ? ) were measured in brain regions of rats during spontaneous morphine withdrawal, which was modeled in male Wistar rats. The animals were injected with the increasing intraperitoneal doses (10–100 mg/kg, twice a day) of morphine hydrochloride for 6 days. Thirty six hours after the last injection the severity of the spontaneous morphine withdrawal syndrome was determined by specific autonomic and locomotor indices The withdrawal was accompanied by the increase of both NOS activity and NO x ? levels in the midbrain and hippocampus, the decrease of these parameters in striatum and hypothalamus, and lack of changes in cerebral cortex and brain stem. In cerebellum NOS activity decreased whereas NO x ? concentrations remained unchanged. In the cerebral cortex, striatum, midbrain, and cerebellum activity of NOS and NO x ? concentrations correlated with the withdrawal syndrome severity and also with the specific signs of abstinence. |
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Keywords: | spontaneous morphine withdrawal nitric oxide nitric oxide synthase nitrate/nitrite concentration brain rats |
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