Identification of ADPR-transferase activity in the rat tapeworm, Hymenolepis diminuta |
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| Authors: | M Alsharif R Apweiler W J Whish C J Branford White R V Brunt |
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| Institution: | Department of Biochemistry, University of Bath, UK. |
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| Abstract: | 1. The nuclear fraction of the rat tapeworm Hymenolepis diminuta (Cestoda) contains the enzyme adenosine diphosphoribosyl transferase (ADPR-transferase). 2. The enzyme catalyzes the postsynthetic modification of some nuclear proteins by the covalent attachment of the (ADP-ribose) moiety of NAD to such proteins. 3. The reaction is dependent on DNA which contains strand-breaks, and chain lengths equivalent to (ADP-ribose) is estimated. 4. The formation of polynucleotide products was competitively inhibited by 3-acetamidobezamide, with a Km of 125 microM. 5. The catalytic properties of ADPR-transferase in Hymenolepis diminuta are similar to those in T. brucei. |
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