Homogeneous cytochrome b5 from human erythrocytes |
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Authors: | D E Hultquist R T Dean R H Douglas |
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Institution: | Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48104, USA |
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Abstract: | Homogeneous cytochrome b5 has been isolated from large volumes of human erythrocytes by sequential chromatography on DEAE-cellulose, Amberlite CG-50, Bio-Gel P-60, and DEAE-Sephadex A-50. A molecular weight of 15,300 was determined by SDS disc gel electrophoresis. Trypsin converted the protein to a smaller hemepeptide which was indistinguishable from trypsin-cytochrome b5 of human liver microsomes by disc gel electrophoresis. The data suggest that erythrocyte cytochrome b5 has the same structure as a segment of liver microsomal cytochrome b5 and is intermediate in size between the trypsin- and detergent-solubilized forms of the liver protein. |
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