猴结核病血清抗体检测方法的建立

目的目前活检猴感染结核病的检疫方法只有结核菌素试验。由于检测方法的单一性,结核菌素试验敏感性和特异性低,给灵长类结核病的管理带来困难。为此,开展了猴结核病血清抗体的检测方法。方法利用胞浆蛋白、结核菌素、Trehalose 6,6-’dimycolate(TDM)分别作为抗原建立猴结核病血清抗体ELISA检测方法,检测了28份自然感染血清和121份阴性血清,评价其应用价值。结果根据三种抗原对标本检测结果的敏感度和特异度,用受试者工作特性曲线(ROC)进行分析比较。胞浆蛋白、结核菌素、TDM三种抗原检测的敏感度分别为89.3%、75%与64.3%;特异度分别为93.4%、95.9%与88.4%。ROC曲线下面积都大于0.5(P<0.01)。结论表明三种抗原能用于抗猴结核病的ELISA检测,具有较好的准确性。其中,胞浆蛋白抗原的敏感性和特异性最高,是猴结核病血清抗体检测较好的一种抗原。

Application of an Enzyme Immunoassay for Detecting Antibody in Serum of Tuberculous Monkeys

Objective Tuberculosis infection in the live monkeys was usually diagnosed by tuberculin test.It was difficult in tuberculous monkeys' management that quarantine method was single,and the sensitivity and specificity of tuberculin test was low.The purpose of this study is to establish an ELISA detection method of mycobacterium antibody in monkeys.Methods Three antigens(cytoplasm protein,PPD-bovis and TDM) were applied in the ELISA test.The application value was evaluated by detecting the sera of 28 monkeys following a natural outbreak of tuberculosis and 121 healthy monkeys.Results On the basis of the sensitivity and specificity of detecting results,they were analyzed by receiver operating characteristic(ROC) curve.The sensitivity of the cytoplasm protein,PPD-bovis,TDM was 89.3%,75% and 64.3%,respectively.The specificity was 93.4%,95.9%,88.4%,respectively.The areas under ROC curve were 0.964,0.858 and 0.844,respectively.All the areas exceeded 0.5,P<0.01.Conclusion These results indicate that all the three antigens can be used in ELISA detection test of serum of tuberculous monkeys.They have good serum diagnostic accuracy.Among them,the cytoplasm protein is a good antigen to detect tuberculous monkeys' antibody,showing the highest sensitivity and specificity in the ELISA tests.The result of ELISA test using TDM in serum-diagnosis of tuberculous monkeys is not satisfactory.