| 长双歧杆菌DD98冻干保护剂优化及菌粉保存稳定性研究 |
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| 引用本文： | 王桃,纪瑞,刘海燕,李忠磊,陈代杰,谭俊.长双歧杆菌DD98冻干保护剂优化及菌粉保存稳定性研究[J].工业微生物,2020(2):22-27. |
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| 作者姓名： | 王桃 纪瑞 刘海燕 李忠磊 陈代杰 谭俊 |
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| 作者单位： | 上海师范大学生命科学学院;上海医药工业研究院;上海交通大学药学院 |
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| 基金项目： | 国家青年科学基金(81603004)。 |
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| 摘 要： | 以长双歧杆菌DD98为研究对象,通过对冻干保护剂配方的优化,冻干菌粉的存活率提高到90%以上。通过进一步稳定性研究,采用保护剂优化配方制备的冻干菌粉在4℃保存24个月后,活菌数仍在1.0×10^10 CFU/g以上,在25℃条件下可以保存12个月,双歧杆菌的存活率在1.0×10^6CFU/g以上,符合FAO/WHO建议食品益生菌活菌数应在1.0×10^6 CFU/g^1.0×10^7CFU/g的标准。
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| 关 键 词： | 双歧杆菌DD98 冻干保护剂 保存稳定性 |
Optimization of freeze-drying protectant for Bifidobacterium longum DD98 and preservation stability of bacterial powder |
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| WANG Tao,JI Rui,LIU Haiyan,LI Zhonglei,CHEN Daijie,TAN Jun.Optimization of freeze-drying protectant for Bifidobacterium longum DD98 and preservation stability of bacterial powder[J].Industrial Microbiology,2020(2):22-27. |
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| Authors: | WANG Tao JI Rui LIU Haiyan LI Zhonglei CHEN Daijie TAN Jun |
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| Institution: | (College of Life Science,Shanghai Normal University,Shanghai 200234,China;Shanghai Institute of Pharmaceutical Industry,Shanghai 201203,China;Shanghai Jiaotong University of Pharmacy,Shanghai 200240,China) |
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| Abstract: | Taking Bifidobacterium longum DD98 as the research object,the survival rate of the lyophilized bacterial powder was increased by more than 90%after optimizing the formula of freeze-drying protective additive.Further stability research showed that when the lyophilized powder prepared by the optimized formula was stored at 4℃for 24 months,the number of viable bacteria in the powder was kept above 1.0×10^10 CFU/g;when the freeze-dried bacterial powder was preserved at 25℃for 12 months,the number of viable bacteria could be stabilized above 1.0×10^6 CFU/g,all of which were in line with the FAO/WHO recommendation that the number of viable bacteria in probiotic food should be 1.0×10^6 CFU/g^1.0×10^7 CFU/g. |
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| Keywords: | Bifidobacterium longum DD98 freeze-drying protective additive preservation stability |
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