Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells |
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| Authors: | Liu Zhen Yang Guan-Zhen Chi Cheng-Wu Wu Xiang-Fu |
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| Affiliation: | Shanghai Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China. |
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| Abstract: | BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated that it was biologically activity. Furthermore, Quantitative estimation show that the biological activity of recombinant BmK ITa1 from Tn cells was more powerful than from E. coli. |
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