Selective separation procedure for determination of ribosomal proteins L7 and L12. |
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| Authors: | K Li A R Subramanian |
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| Institution: | 1. Biochemical Research Laboratory, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;2. the Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02114 USA |
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| Abstract: | An electrophoretic procedure for the selective separation and determination of the closely similar ribosomal proteins L7 and L12 (which are specifically involved in the GTPase reactions of the ribosome) from the total protein mixture extracted from unwashed ribosomes is described. In this procedure, which takes advantage of their unusually low isoelectric points. L7 and L12 (and a few other acidic proteins) migrate into gel asanions, while the bulk of ribosomal proteins which are basic remain behind. The positions of L7 and L12 were determined with authentic, pure proteins. It was further determined by means of 2-dimensional gel electrophoresis that no other protein components present in unwashed ribosomes comigrate with the bands of L7 and L12. |
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| Keywords: | To whom correspondence should be addressed Presently at: Max-Planck-Institut fu¨r Molekulare Genetik Berlin 33 (Dahlem) W Germany |
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